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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in many tumors, which leads to chromosome instability, and therefore could contribute to tumorigenesis. This protein was uncovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a important position in advertising and marketing mobile-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have already been explained. Two extra splice variants have been documented but comprehensive nucleotide sequence information and facts is just not however out there.
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Blocking peptides are peptides that bind specifically on the concentrate on antibody and block antibody binding. These peptide generally has the epitope regarded through the antibody.
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This guidebook outlines additional attainable optimization of mobile and immunoassay parameters to be sure the very best effects are...
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This tutorial outlines further more probable optimization of mobile and immunoassay parameters to ensure the best possible results are...
Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By evaluating the staining within the blocked antibody versus the antibody alone, one can see which staining is specific.
Target Data Cyclin E belongs for the remarkably conserved cyclin spouse and children, whose customers are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation styles which lead to the temporal coordination of each and every mitotic function. Cyclin E forms a posh with and functions to be a regulatory subunit of CDK2, whose activity is needed for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells progress via S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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